MIP-2 Viral Recombinant ( MIP2 Viral )

Data sheet

Formulation

Lyophilized from 1mg/ml solution containing 20mM Phospahte Buffer, pH 7.4 % 0.15M NaCl.

Solubility

It is recommended to reconstitute the lyophilized CXCL2 Viral in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 97.0% as determined by RP-HPLC & SDS-PAGE.

Description

MIP-2 Viral Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.9 kDa. The MIP-2 is purified by proprietary chromatographic techniques.

Protein Background

Viral MIP-2 is closely related to MIP-1α, show amino acid sequence similarity of about 41%. At the amino acid sequence level, Viral MIP-1 and Viral MIP-2 share 48% similarity. Viral MIP-1 and Viral MIP-2 are more closely linked to one another phyloge

Expression host

Escherichia Coli.

Synonyms

MIP-2 Viral, Viral MIP-2, MIP2 Viral, Viral MIP2, Viral Macrophage inflammatory Protein-2.

Stability

Lyophilized MIP-2 Viral protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recomm

Amino acid sequence

LGASWHRPDK CCLGYQKRPL PQVLLSSWYP TSQLCSKPGV IFLTKRGRQVCADKSKDWVK KLMQQLPVTA.

Physical Appearence

Sterile Filtered White lyophilized (freeze-dried) powder.

Biological Activity

Determined by the inhibitory effect on monocyte migration response to human MIP1A using a concentration range of 1ug-10ug/ml of viral MIP2 which will inhibit 25ng/ml of human MIP1A.