Interleukin-10 Mouse Recombinant ( IL 10 Mouse )

Data sheet

Reconstitution

In PBS, pH 7.4 and 0.1 % NaN3.

Formulation

The protein was lyophilized after extensive dialysis against PBS.

Solubility

It is recommended to reconstitute the lyophilized IL-10 Mouse Recombinant in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

IL-10 Recombinant Mouse produced in E.coli is a single, non-glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18785 Dalton. The Interleukin-10 Mouse is purified by proprietary chromatographic techniques.

Protein Background

IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory mo

Expression host

Escherichia Coli.

Synonyms

B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized IL-10 Mouse although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Interleukin10 Mouse recombinant should be stored at 4°C between 2-7 days and for future use below -18°C.For long term sto

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Arg-Gly-Gln.

Biological Activity

The ED50 as determined by the dose-dependant co-stimulation with IL-4 of mouse MC-9 cells was found to be

Titer

Antibody is shipped as in liquid form with ice packs.