Interleukin-13 Mouse Recombinant ( IL 13 Mouse )

Data sheet

Reconstitution

8.6mg/ml.

Formulation

The protein (1mg/ml) was lyophilized in PBS, pH 7.2 and 5% trehalose.

Solubility

It is recommended to reconstitute the lyophilized Interleukin 13 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95% as determined by SDS-PAGE.

Description

Interleukin-13 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 111 amino acids and having a molecular mass of 12.3 kDa. The IL-13 is purified by proprietary chromatographic techniques.

Protein Background

IL13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. IL-13 is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells.

Expression host

Escherichia Coli.

Synonyms

Interleukin-13, NC300, ALRH, BHR1, P600, IL-13, IL13.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Interleukin-13 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL13 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended t

Amino acid sequence

MPVPRSVSLP LTLKELIEEL SNITQDQTPL CNGSMVWSVD LAAGGFCVAL DSLTNISNCN AIYRTQRILH GLCNRKAPTT VSSLPDTKIE VAHFITKLLS YTKQLFRHGP F.

Biological Activity

The ED50 range=4 ng/ml, corresponding to a specific activity of 250,000IU/mg as determined by the dose dependent proliferation of TF-1 cells.

Product Description

Insulin, human.

Antibody Content

Kass for clone 7F8 is 7.5 x 109M-1 These antibodies cross-react with human proinsulin, bovine insulin (30%) and porcine insulin. No cross-reaction with free C-peptide.

Presentation

IgG1 kappa.

Purification

The vial contains antibody in PBS, pH 7.4, containing 0.1% of sodium azide as preservative.