Interleukin-2 Rat Recombinant ( IL 2 Rat )

Data sheet

Formulation

The protein (1mg/ml) was lyophilized without any additives.

Solubility

It is recommended to reconstitute the lyophilized Interleukin 2 in sterile 100mM AcOH not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

Interleukin-2 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 17kDa. The IL-2 is purified by proprietary chromatographic techniques.

Protein Background

IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of

Expression host

Escherichia Coli.

Synonyms

T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Interleukin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Ser.

Biological Activity

The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is 0.1-0.4 ng/ml corresponding to a specific activity of 2,500,000-10,000,000 units/mg.

References

Title:Functional Gap Junctions Facilitate Melanoma Antigen Transfer and Cross-Presentation between Human Dendritic Cells.Publication:The Journal of Immunology June 1, 2007 vol. 178 no. 11 6949-6957 Link:http://www.jimmunol.org/content/178/11/6949.full