Interleukin-3 Human Recombinant ( IL 3 Human )

Data sheet

Formulation

Lyophilized from a concentrated (1mg/ml) solution in water containing 0.3 mg/ml of NaHCO3.

Solubility

It is recommended to reconstitute the lyophilized Interleukin-3 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE.

Description

Interleukin-3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton. The IL-3 is purified by proprietary chromatographic techniques.

Protein Background

IL3 is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytok

Expression host

Escherichia Coli.

Synonyms

MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL3 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Thr-Gln.

Biological Activity

The ED50 as determined by the dose-dependant stimulation of TF-1 cells is

References

1.Title:Interleukin-10 overexpression in macrophages suppresses atherosclerosis in hyperlipidemic mice.Publication:Published online before print March 30, 2010, doi: 10.1096/fj.09-148155 August 2010 The FASEB Journal vol. 24 no. 8 2869-2880 .Link:http://w