Interleukin-3 Mouse Recombinant ( IL 3 Mouse )

Data sheet

Formulation

Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.

Solubility

It is recommended to reconstitute the lyophilized Interleukin-3 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 97.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE.

Description

Interleukin-3 mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 135 amino acids and having a molecular mass of 15100 Dalton. The IL-3 is purified by proprietary chromatographic techniques.

Protein Background

Interleukin-3 is a pleiotropic cytokine produced primarily by activated T cells. IL-3 is thought to function via specific cell surface receptors to stimulate the proliferation, differentiation and survival of haematopoietic cell lines. IL-3 has also been

Expression host

Escherichia Coli.

Synonyms

MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-3 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Asp-Thr-His-Arg.

Biological Activity

The ED50 as determined by the dose-dependant stimulation of murine M-NFS-60 cells is

References

1.Title:Direct binding of Cbl to Y568 and Y936 of the stem cell factor receptor/c-Kit is required for ligand-induced ubiquitination, internalization and degradation.Publication:Biochemical Journal Immediate Publication. Published on 19 Jun 2006 as manuscr