Interleukin-4 Mouse Recombinant ( IL 4 Mouse )

Catalog # PT_41199

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$201.00
DescriptionInterleukin-4 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 120 amino acids and having a molecular mass of 13500 Dalton. The IL-4 is purified by proprietary chromatographic
Product Size: 5ug
Quantity

Data sheet

Formulation
Lyophilized from a 0.2um concentrated (1mg/ml) solution in PBS pH 7.4.
Solubility
It is recommended to reconstitute the lyophilized Interleukin 4 in sterile 10mM HAc not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Purity
Greater than 96.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Description
Interleukin-4 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 120 amino acids and having a molecular mass of 13500 Dalton. The IL-4 is purified by proprietary chromatographic techniques.
Protein Background
Interleukin-4 is a pleiotropic cytokine produced primarily by activated T lymphocytes, basophils and mast cells. Multiple immune response-modulating functions are performed by IL-4 on a variety of cell types and it has an important role in the regulator o
Expression host
Escherichia Coli.
Synonyms
BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.
Reagent Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Stability
Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL4 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to
Amino acid sequence
MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVCRASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNESKSTSLKDFLE SLKSIMQMDY S.
Biological Activity
The ED50 as determined by the dose-dependant induction of HT-2 cell proliferation is less than 2 ng/ml corresponding to a Specific Activity of 500,000IU/mg.
References
Title: CD26, adenosine deaminase, and adenosine receptors mediate costimulatory signalsin the immunological synapse.publication: Published online before printJune 27, 2005, doi:10.1073/pnas.0501050102PNASJuly 5, 2005vol. 102no. 279583-9588.Link: http://ww
Quality Control Test
MEGVHRKPSF LALPGHLVKS EETVILQCWS DVMFEHFLLH REGKFNNTLH LIGEHHDGVS KANFSIGPMM PVLAGTYRCY GSVPHSPYQL SAPSDPLDMV IIGLYEKPSL SAQPGPTVQA GENVTLSCSS RSSYDMYHLS REGEAHERRL PAVRSINGTF QADFPLGPAT HGGTYRCFGS FRDAPYEWSN SSDPLLVSVT GN.
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