Interleukin-7 Human Recombinant, Yeast ( IL 7 Human, Yeast )

Data sheet

Formulation

Lyophilized from a concentrated (1mg/ml) solution in water containing 20mM phosphate buffer.

Solubility

It is recommended to reconstitute the lyophilized Interleukin -7 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 98.0% as determined by SDS-PAGE.

Description

Interleukin-7 Human Recombinant produced in yeast is a single, glycosylated polypeptide chain containing 152 amino acids and having a molecular mass of 17.4 kDa. The IL-7 is purified by proprietary chromatographic techniques.

Protein Background

IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangemen

Expression host

Saccharomyces cerevisiae.

Synonyms

Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL7 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Cys-Asp-Ile-Glu.

Biological Activity

The ED50 as determined by the dose-dependent stimulation of thymidine uptake by murine pre-B cell line 2E8 is 2 x 106 units/mg.