Interleukin-7 Mouse Recombinant ( IL 7 Mouse )

Data sheet

Formulation

Filtered (0.2um) and lyophilized from a concentrated (1mg/ml) solution in 1PBS, pH7.4 and 2% trehalose.

Solubility

It is recommended to reconstitute the lyophilized Interleukin 7 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 96.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

Interleukin-7 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 129 amino acids and having a molecular mass of 14.9kDa.

Protein Background

Interleukin-7 is a potent lymphoid cell growth factor produced primarily by stromal cells. IL-7 has been shown to support the proliferation and differentiation of pre B- and early T cells as well as displaying a biological effect on cells of NK and myeloi

Expression host

Escherichia Coli.

Synonyms

Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL7 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to

Amino acid sequence

ECHIKDKEGK AYESVLMISI DELDKMTGTD SNCPNNEPNF FRKHVCDDTK EAAFLNRAAR KLKQFLKMNI SEEFNVHLLT VSQGTQTLVN CTSKEEKNVK EQKKNDACFL KRLLREIKTC WNKILKGSI.

Biological Activity

The ED50was determined by the dose-dependent stimulation of the proliferation of murine 2E8 cells is less than 0.2 ng/ml, corresponding to a specific activity of >5.0106 IU/mg.

References

Title: Disruption of Bis Leads to the Deterioration of the Vascular Niche for Hematopoietic Stem CellsPublication: Stem Cells 28.2 (2010): 268-278.Link: http://onlinelibrary.wiley.com/doi/10.1002/stem.285/full