Interleukin-10 Human Recombinant, HEK ( IL 10 Human, HEK )

Data sheet

Formulation

The IL10 was lyophilized from 1mg/ml in 10mM Tris-HCl, pH 7.4 and 150mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized IL-10 in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95% as obsereved by SDS-PAGE.

Description

Interleukin-10 Human Recombinant produced in HEK cells is a glycosylated non-disulfide bonded homodimer, having a total molecular weight of 17kDa.The IL10 is purified by proprietary chromatographic techniques.

Protein Background

IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory mo

Expression host

HEK.

Synonyms

B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.

Stability

Lyophilized IL-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL10 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a c

Physical Appearence

Sterile Filtered White lyophilized (freeze-dried) powder.

Biological Activity

The specific activity was determined by the dose-dependent stimulation of the proliferation of MC/9 cells (mouse mast cell line) in the presence of 200 pg/ml IL-4, and is typically 0.3-1.5ng/ml.