Interleukin-13 Variant Human Recombinant ( IL 13 Variant Human )

Catalog # PT_41239

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$201.00
DescriptionInterleukin-13 Variant Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 114 amino acids, with a substitution of Q for R at position 112 compared with the wild type IL-13, having a molecular
Product Size: 2ug
Quantity

Data sheet

Formulation
Lyophilized from a 0.2um filtered concentrated solution in 1xPBS, pH 7.2, containing 5% trehalose.
Solubility
It is recommended to reconstitute the lyophilized Interleukin 13 Variant in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Purity
Greater than 95% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Description
Interleukin-13 Variant Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 114 amino acids, with a substitution of Q for R at position 112 compared with the wild type IL-13, having a molecular mass of 12.5 kDa.
Protein Background
IL13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. IL-13 is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells.
Expression host
Escherichia Coli.
Synonyms
Interleukin-13, NC30, ALRH, BHR1, P600, IL-13, MGC116786, MGC116788, MGC116789.
Reagent Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Stability
Lyophilized Interleukin-13 Variant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL13 Variant should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it
Amino acid sequence
SPGPVPPSTA LRELIEELVN ITQNQKAPLC NGSMVWSINL TAGMYCAALE SLINVSGCSA IEKTQRMLSG FCPHKVSAGQ FSSLHVRDTK IEVAQFVKDL LLHLKKLFRE GQFN.
Biological Activity
The ED50 was determined by the dose dependent prolifiration of TF-1 cells and was found to be 1,000,000 units/mg. This analog has also been shown to exhibit increased in vivo activity compared to wild type IL-13.
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