Interleukin-21 Mouse Recombinant ( IL 21 Mouse )

Data sheet

Formulation

Mouse IL-21 was lyophilized from 20mM NaHCO3, pH 8.5.

Solubility

It is recommended to reconstitute the lyophilized Mouse IL21 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

Interleukin-21 Mouse Recombinant produced in E.Coli is a single, non-glycosilated polypeptide chain containing 130 amino acids and having a total molecular mass of 15kDa. The Murine IL-21 is purified by proprietary chromatographic techniques.

Protein Background

IL-21 is produced by CD4+ T cells in response to antigenic stimulation. Its action enhances antigen-specific responses of immune cells. The biological effects of IL-21 include induction of differentiation of T-cells-stimulated B-cells into plasma cells an

Expression host

Escherichia Coli.

Synonyms

Interleukin-21, IL-21, Il21.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Mouse IL-21 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Mouse IL21 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommend

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Lys-Ser-Ser.

Biological Activity

The ED50=10 -50 ng/ml, determined by the cell proliferation assay of modified Ba/F3 cells.