Interleukin-2 Mouse Recombinant, His Tag ( IL 2 Mouse, His )

Data sheet

Formulation

The Mouse IL2 protein (0.5mg/ml) is supplied in Phosphate Buffered Saline (pH7.4) 0.1M NaCl, and 20% glycerol.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Description

Interleukin-2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 170 amino acids (21-169 a.a) and having a molecular mass of 19.5kDa. The mouse IL-2 is fused to a 20 amino acid His tag at N-Terminus an

Protein Background

IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of

Expression host

Escherichia Coli.

Synonyms

Interleukin-2, T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2, Il2.

Reagent Appearance

Sterile Filtered colorless clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MAPTSSSTSS STAEAQQQQQ QQQQQQQHLE QLLMDLQELL SRMENYRNLK LPRMLTFKFY LPKQATELKD LQCLEDELGP LRHVLDLTQS KSFQLEDAEN FISNIRVTVV KLKGSDNTFE CQFDDESATV VDFLRRWIAF CQSVISTSPQ.

Biological Activity

Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is