Lymphatic Vessel Endothelial Hyaluronic Acid Receptor 1 Human Recombinant, Sf9 ( LYVE1 Human Sf9 )

Data sheet

Formulation

LYVE1 was lyophilized from a concentrated (1 mg/ml) sterile solution containing no additives.

Solubility

It is recommended to reconstitute the lyophilized LYVE1 in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95.0% as determined by(a)Analysis by RP-HPLC.(b)Analysis by SDS-PAGE.

Description

Soluble LYVE1 Human Recombinant fused to a C-terminal His-tag (6xHis) produced in baculovirus is a monomeric, glycosylated, polypeptide containing 232 amino acids (Met-1 to Gly 232) and having a total molecular mass of 25 kDa but as a result of glycosilat

Protein Background

LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD

Expression host

Insect Cells.

Synonyms

Lymphatic vessel endothelial hyaluronic acid receptor 1 precursor, LYVE-1, Cell surface retention sequence-binding protein 1, CRSBP-1, Hyaluronic acid receptor, Extracellular link domain-containing protein 1.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized sLYVE-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution sLYVE-1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to ad

5X Reaction Buffer

5-20ug per ml for In-Vitro Experiments and 50-100ug per animal for In-Vivo study.The protein can be used for T-cell proliferation, cytokine induction, antigen presentation, western blotting, ELISA and EAE induction in mice.