Macrophage Inflammatory Protein-3 Beta Mouse Recombinant ( CCL19 ) ( MIP 3b Mouse )

Data sheet

Formulation

The CCL19 was lyophilized from a concentrated (1mg/ml) solution in water containing no additives.

Solubility

It is recommended to reconstitute the lyophilized Macrophage Inflammatory Protein-3b in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

Macrophage Inflammatory Protein-3 beta Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 83 amino acids and having a molecular mass of 9216 Dalton. The MIP-3b is purified by proprietary chromatographic techni

Protein Background

Chemokine (C-C motif) ligand 19 (CCL19) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta). CCL19 is expressed abundantly in thymus and lymph n

Expression host

Escherichia Coli.

Synonyms

Small inducible cytokine A19, CCL19, Macrophage inflammatory protein 3 beta, MIP-3- beta, EBI1-ligand chemokine, ELC, Beta chemokine exodus-3, CK beta-11, chemokine (C-C motif) ligand 19, CKb11, MIP3B, MIP-3b, SCYA19, MGC34433, Epstein-Barr virus-induced

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized MIP-3b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL19 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be, Gly-Ala-Asn-Asp-Ala.

Biological Activity

The Activity is calculated by the ability to chemoattract Human mature dendritic cells using a concentration range of 8-80 ng/ml.