Protein Disulfide Isomerase Human Recombinant ( PDI Human )

Data sheet

Formulation

The PDI protein (1mg/ml)solution was lyophilized from PBS pH-7.

Solubility

It is recommended to reconstitute the lyophilized PDI in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95.0% as determined bya) Analysis by RP-HPLC.b) Analysis by SDS-PAGE.

Description

PDI Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 503 amino acids and having a molecular mass of 62.4 kDa. The PDI is fused to a 12 amino acid His tag (515 a.a. total) at N-terminal and purified by propr

Protein Background

Protein disulfide isomerases (PDIs) constitute a family of structurally related enzymes which catalyze disulfide bonds formation, reduction, or isomerization of newly synthesized proteins in the lumen of the endoplasmic reticulum (ER). They act also as ch

Expression host

Escherichia Coli.

Synonyms

Protein Disulfide Isomerase, PDI, EC 5.3.4.1, Prolyl 4-hydroxylase subunit beta, Cellular thyroid hormone-binding protein, p55, P4HB, ERBA2L, PDIA1, PO4DB, DSI, GIT, PHDB, PO4HB, PROHB, P4Hbeta.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Protein Disulfide Isomerase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Human PDI should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Arg-Ala.

References

Title: Protein disulfide isomerases are antibody targets during immune-mediated tumor destruction Publications: Blood 113.8 (2009): 1681-1688. Link: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2647666/