Rantes Human Recombinant ( CCL5 ) ( Rantes Human )

Data sheet

Formulation

Lyophilized from a 0.2um filtered concentrated solution in 50mM NaAc, pH 5.0, 100mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized Rantes in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

Rantes Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 68 amino acids and having a molecular mass of 7809.2 Dalton. The Rantes is purified by proprietary chromatographic techniques.

Protein Background

Regulated upon Activation, Normal T-cell Expressed, and Secreted or RANTES is an 8 kDa protein classified as a chemotactic cytokine or chemokine. Rantes has recently been renamed CCL5. RANTES is chemotactic for T cells, eosinophils and basophils and plays

Expression host

Escherichia Coli.

Synonyms

Small inducible cytokine A5, CCL5, T-cell-specific RANTES protein, SIS-delta, T cell- specific protein P228, TCP228, chemokine (C-C motif) ligand 5, SISd, SCYA5, RANTES, D17S136E, MGC17164.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Rantes although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Rantes should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add

Amino acid sequence

SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS.

Biological Activity

The Rantes activity is determined by the chemoattract of human blood monocytes at a concentration between 1-10ng/ml corresponding to a Specific Activity of 100,000-1,000,000IU/mg.