TNF Ligand Superfamily Member 12 Human Recombinant ( TNFSF12 Human )

Data sheet

Formulation

Filtered and lyophilized from 0.5mg/ml in 20mM Tris buffer and 50mM NaCl pH-7.5.

Solubility

Add pyrogen free water to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Purity

Greater than 90% as determined by SDS-PAGE.

Description

The TWEAK Human Recombinant is manufactured with N-terminal fusion of 13 amino acid FLAG Tag. The TNFSF12 Flag -Tagged Fusion Protein has a Mw of 18.7kDa containing a total of 169 amino acid residues.

Protein Background

TNFSF12 is a cytokine that is part of the TNF ligand family. TNFSF12 is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. TNFSF12 induces apoptosis through multiple

Expression host

HEK293.

Synonyms

TWEAK, TNF-related weak inducer of apoptosis, TNFSF12, DR3LG, Apo3-Ligand, APO3L, TNFRSF12A, Tumor necrosis factor ligand superfamily member 12, MGC20669, MGC129581.

Reagent Appearance

Filtered White lyophilized (freeze-dried) powder.

Stability

Store lyophilized TWEAK Human recombinant at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at

Amino acid sequence

HVDYKDDDDK PAGSAPKGRK TRARRAIAAH YEVHPRPGQD GAQAGVDGTV SGWEEARINS SSPLRYNRQI GEFIVTRAGL YYLYCQVHFD EGKAVYLKLD LLVDGVLALR CLEEFSATAA SSLGPQLRLC QVSGLLALRP GSSLRIRTLP WAHLKAAPFL TYFGLFQVH.