More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| kinase insert domain receptor(a type III receptor tyrosine kinase) | Monoclonal | IgG | Mouse | Human | IHC-F | Ascites |
Immunogen |
| Recombinant human extracellular domain of VEGFR-2(KDR). |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | KDR |
Protein | Vascular endothelial growth factor receptor 2 |
Uniprot ID | Human(P35968), Mouse(P35918), Rat(O08775) |
Function | Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC. |
Tissue Specificity | Detected in cornea (at protein level). Widely expressed. |
Sub-cellular localization | Cell junction . Endoplasmic reticulum. |
Sequence Similarities | Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. |
Aliases | CD309|VEGFR|FLK1|VEGFR2|TYROSINE KINASE GROWTH FACTOR RECEPTOR|FLK1, MOUSE, HOMOLOG OF|VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR|VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR 2|Fetal liver kinase 1|Kinase insert domain receptor|Protein-tyrosine kinase receptor flk-1 |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Immunohistochemistry(Frozen Section) | 1-2μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
