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S-100(beta-subunit) Antibody (monoclonal) View larger

S-100(beta-subunit) Antibody (monoclonal)

ASA-B1643

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
S100 calcium binding protein B Monoclonal IgG Mouse Human, Rabbit, Rat IHC-P Ascites

Immunogen

Bovine brain S-100b.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.

Concentration

 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 S100B

Protein

 Protein S100-B

Uniprot ID

 Human(P04271), Mouse(P50114), Rat(P04631)

Function

 Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.

Tissue Specificity

Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.

Sub-cellular localization

 Cytoplasm.

Sequence Similarities

 Belongs to the S-100 family.

Aliases

 NEF|S100|S100-B|S100beta|S100 calcium binding protein, beta(neural)|S-100 protein beta chain|S-100 protein subunit beta

Application Details

Application Concentration* Species Validated Using**
Immunohistochemistry(Paraffin-embedded Section)1-2μg/mlHuman, rabbit, ratAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-S-100(beta-subunit) antibody (monoclonal), ASA-B1643, IHC(P)
IHC(P): Rat Brain Tissue Lysate

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