More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| S100 calcium binding protein B | Monoclonal | IgG | Mouse | Human, Rabbit, Rat | IHC-P | Ascites |
Immunogen |
| Bovine brain S-100b. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | S100B |
Protein | Protein S100-B |
Uniprot ID | Human(P04271), Mouse(P50114), Rat(P04631) |
Function | Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity. |
Tissue Specificity | Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the S-100 family. |
Aliases | NEF|S100|S100-B|S100beta|S100 calcium binding protein, beta(neural)|S-100 protein beta chain|S-100 protein subunit beta |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Immunohistochemistry(Paraffin-embedded Section) | 1-2μg/ml | Human, rabbit, rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-S-100(beta-subunit) antibody (monoclonal), ASA-B1643, IHC(P)
IHC(P): Rat Brain Tissue Lysate
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