Glucagon Antibody (monoclonal)

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
glucagon	Monoclonal	IgG	Mouse	Human, Mouse, Rabbit, Rat	IHC-P	Ascites
Immunogen
Polymerized porcine glucagon.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.
Concentration	1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	GCG
Protein	Glucagon
Uniprot ID	Human(P01275), Mouse(P55095), Rat(P06883)
Function	Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.
Tissue Specificity	Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP-1 and GLP-2 are also secreted in selected neurons in the brain.
Sub-cellular localization	Secreted.
Sequence Similarities	Belongs to the glucagon family.
Aliases	GLP1|GLP2|glicentin-related polypeptide|glucagon-like peptide 1|glucagon-like peptide 2

Application Details

Application	Concentration*	Species	Validated Using**
Immunohistochemistry(Paraffin-embedded Section)	1-2μg/ml	Human, mouse, rat, rabbit	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information