More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| mucin 1, cell surface associated | Monoclonal | IgG | Mouse | Human | IHC-P, IHC-F | Ascites |
Immunogen | ||||||
| Human milk fat globule membranes. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MUC1, MUC1 |
Protein | Mucin-1 |
Uniprot ID | Human(15941), Mouse(02496), Rat(B2GV31) |
Function | The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. |
Tissue Specificity | Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform 7 is expressed in tumor cells only. |
Sub-cellular localization | Apical cell membrane. |
Sequence Similarities | Contains 1 SEA domain. |
Aliases | CD227|EMA|PEM|PEMT|KL-6|PUM|MUCIN 1, URINARY|Episialin|H23AG|PEANUT-REACTIVE URINARY MUCIN|MUCIN, TUMOR-ASSOCIATED EPITHELIAL|POLYMORPHIC EPITHELIAL MUCIN|EPITHELIAL MEMBRANE ANTIGEN|mucin 1, transmembrane|Breast carcinoma-associated antigen DF3|Carcinoma-associated mucin|Krebs von den Lungen-6|Peanut-reactive urinary mucin|Polymorphic epithelial mucin|Tumor-associated epithelial membrane antigen|Tumor-associated mucin |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Immunohistochemistry(Paraffin-embedded Section) | 2-4μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 2-4μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
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Anti- Episialin, EMA antibody, ASA-B0660, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
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Anti- Episialin, EMA antibody, ASA-B0660, IHC(P)
IHC(P): Human Lung Cancer Tissue
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Anti- Episialin, EMA antibody, ASA-B0660, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue