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Collagen, Type IV Antibody (monoclonal) View larger

Collagen, Type IV Antibody (monoclonal)

ASA-B0478

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
collagen, type IV, alpha-1; collagen, type IV, alpha 3(Goodpasture antigen); collagen,type IV, alpha 4; collagen, type IV, alpha 5; collagen, type IV, alpha 6 Monoclonal IgG Mouse Human IHC-P, IHC-F Ascites

Immunogen

Human collagen type IV.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.

Concentration

 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 COL4A1; COL4A3; COL4A4; COL4A5; COL4A6

Protein

 Collagen alpha-1(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; Collagen alpha-5(IV) chain; Collagen alpha-6(IV) chain

Uniprot ID

 Human(P02462), Mouse(P02463), Rat(Q5FWY9), Human(Q01955), Mouse(Q9QZS0), Rat(F1LRJ1), Human(P53420), Mouse(Q9QZR9), Rat(Q5U1X9), Human(P29400), Mouse(Q63ZW6), Rat(F1LUN5), Human(Q14031), Mouse(B1AVK5)

Function

 Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.

Tissue Specificity

Highly expressed in placenta.

Sub-cellular localization

 Secreted, extracellular space, extracellular matrix, basement membrane.

Sequence Similarities

 Belongs to the type IV collagen family.

Aliases

 ICH|HANAC|POREN1|arresten

Application Details

Application Concentration* Species Validated Using**
Immunohistochemistry(Paraffin-embedded Section)0.5-1μg/mlHumanAssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section)0.5-1μg/mlHumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Collagen, Type IV antibody (monoclonal), ASA-B0478, IHC(P)
IHC(P): Human Mammary Cancer Tissue

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