More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| CD8a molecule; CD8b molecule | Monoclonal | IgG | Mouse | Human | IHC-F | Ascites |
Immunogen |
| Human thymocytes followed by peripheral blood T cells. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CD8A; CD8B |
Protein | T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain |
Uniprot ID | Human(P01732), Mouse(P01731), Rat(P07725), Human(P10966), Mouse(P10300), Rat(P05541) |
Function | Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. |
Tissue Specificity | |
Sub-cellular localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. |
Sequence Similarities | Contains 1 Ig-like V-type (immunoglobulin-like) domain. |
Aliases | CD8|MAL|p32|Leu2|CD8B1|LY3|LYT3|T-lymphocyte differentiation antigen T8/Leu-2 |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Immunohistochemistry(Frozen Section) | 1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
