Zonula occludens protein 3 Antibody View larger

Zonula occludens protein 3 Antibody

ASA-B2008

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

tight junction protein 3 Polyclonal IgG Rabbit Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Zonula occludens protein 3(885-905aa RARDVESSDDEGYDWGPATDL), identical to the related rat sequence.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

TJP3

Protein

Tight junction protein ZO-3

Uniprot ID

Q9QXY1

Function

Tissue Specificity

Sub-cellular localization

Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, tight junction .

Sequence Similarities

Belongs to the MAGUK family.

Aliases

Tight junction protein 3 antibody|Tight junction protein ZO-3 antibody|Tight junction protein ZO3 antibody|TJP3 antibody|ZO3 antibody|ZO3_HUMAN antibody|Zona occludens protein 3 antibody|Zonula occludens protein 3 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Rat MouseAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Mouse, Rat MouseAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Zonula occludens protein 3 antibody, ASA-B2008, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Heart Tissue Lysate
Anti-Zonula occludens protein 3 antibody, ASA-B2008, IHC(P)
IHC(P): Rat Intestine Tissue
Anti-Zonula occludens protein 3 antibody, ASA-B2008, IHC(P)
IHC(P): Mouse Liver Tissue

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