More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| X-ray repair complementing defective repair in Chinese hamster cells 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human XRCC1(15-34aa QDSTHCAENLLKADTYRKWR), identical to the related mouse sequence, and different from the related rat sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | XRCC1 |
Protein | DNA repair protein XRCC1 |
Uniprot ID | P18887 |
Function | Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Contains 2 BRCT domains. |
Aliases | DNA repair protein XRCC1 antibody|RCC antibody|X ray repair complementing defective repair in chinese hamster antibody|X ray repair complementing defective repair in chinese hamster cells 1 antibody|X ray Repair Complementing Defective Repair in Chinese Hamster Cells antibody|X ray repair cross complementing protein 1 antibody|X ray repair, complementing defective, repair in Chinese hamster antibody|X-ray repair cross-complementing protein 1 antibody|XRCC 1 antibody|Xrcc1 antibody|XRCC1_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-XRCC1 antibody, ASA-B1999, Western blotting
Lane 1: 293T Cell Lysate
Lane 2: A431 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 1: 293T Cell Lysate
Lane 2: A431 Cell Lysate
Lane 3: HELA Cell Lysate
Anti-XRCC1 antibody, ASA-B1999, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
Anti-XRCC1 antibody, ASA-B1999, ICC
ICC: A549 Cell
ICC: A549 Cell