More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| wingless-type MMTV integration site family, member 2B | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Wnt2b (87-112 aa QRYPDIMRSVGEGAREWIRECQHQFR), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | WNT2B |
Protein | Protein Wnt-2b |
Uniprot ID | Q93097 |
Function | Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis. |
Tissue Specificity | Isoform 1 is expressed in adult heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the adult brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidney. Isoform 2 is expressed in fetal brain, fetal lung, fetal kidney, caudate nucleus, testis and cancer cell lines. |
Sub-cellular localization | Secreted, extracellular space, extracellular matrix. |
Sequence Similarities | |
Aliases | Protein Wnt-13 antibody|Protein Wnt-2b antibody|Wingless type MMTV integration site family, member 13 antibody|Wingless type MMTV integration site family, member 2B antibody|WNT13 antibody|Wnt2b antibody|WNT2B_HUMAN antibody| XWNT2 antibody|XWNT2, Xenopus, homolog of antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- WNT2B antibody, ASA-B1990, Western blotting
All lanes: Anti WNT2B (ASA-B1990) at 0.5ug/ml
WB: 22RV1 Whole Cell Lysate at 40ug
Predicted bind size: 44KD
Observed bind size: 44KD
All lanes: Anti WNT2B (ASA-B1990) at 0.5ug/ml
WB: 22RV1 Whole Cell Lysate at 40ug
Predicted bind size: 44KD
Observed bind size: 44KD
Anti- WNT2B antibody, ASA-B1990, IHC(P)
IHC(P): Mouse Brain Tissue
IHC(P): Mouse Brain Tissue
Anti- WNT2B antibody, ASA-B1990, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue