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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| Wiskott-Aldrich syndrome | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human WASP (129-156aa ADEDEAQAFRALVQEKIQKRNQRQSGDR), different from the related mouse sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | WAS |
Protein | Wiskott-Aldrich syndrome protein |
Uniprot ID | P42768 |
Function | Effector protein for Rho-type GTPases. Regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. |
Tissue Specificity | Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen. |
Sub-cellular localization | Cytoplasm, cytoskeleton. |
Sequence Similarities | Contains 1 CRIB domain. |
Aliases | Eczema thrombocytopenia antibody|IMD2 antibody|SCNX antibody|THC antibody| THC1 antibody| Thrombocytopenia 1 (X linked) antibody|U42471 antibody|Was antibody|WAS protein antibody| WASp antibody|WASP_HUMAN antibody|Wiskott Aldrich syndrome (eczema thrombocytopenia) antibody|Wiskott Aldrich syndrome (eczema thrombocytopenia) protein antibody|Wiskott Aldrich syndrome antibody| Wiskott Aldrich syndrome protein antibody|Wiskott-Aldrich syndrome protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- WASP antibody, ASA-B1985, Western blotting
All lanes: Anti WASP (ASA-B1985) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: Rat Pancreas Tissue Lysate at 50ug
Lane 5: HEPG2 Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: HEPA Whole Cell Lysate at 40ug
Lane 8: 22RV1 Whole Cell Lysate at 40ug
Predicted bind size: 53KD
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All lanes: Anti WASP (ASA-B1985) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: Rat Pancreas Tissue Lysate at 50ug
Lane 5: HEPG2 Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: HEPA Whole Cell Lysate at 40ug
Lane 8: 22RV1 Whole Cell Lysate at 40ug
Predicted bind size: 53KD
O
Anti- WASP antibody, ASA-B1985, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
Anti- WASP antibody, ASA-B1985, IHC(F)
IHC(F): Human Placenta Tissue
IHC(F): Human Placenta Tissue