More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| von Willebrand factor | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human VWF recombinant protein (Position: R2535-K2813). Human VWF shares 79% amino acid (aa) sequence identity with mouse VWF. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | VWF |
Protein | von Willebrand factor |
Uniprot ID | P04275 |
Function | Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. |
Tissue Specificity | Plasma. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Contains 1 CTCK (C-terminal cystine knot-like) domain. |
Aliases | Coagulation factor VIII antibody|Coagulation factor VIII VWF antibody|F8VWF antibody|Factor VIII related antigen antibody|von Willebrand antigen 2 antibody|Von Willebrand antigen II antibody|Von Willebrand disease antibody|VWD antibody|VWF antibody|VWF_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-VWF antibody, ASA-B1983--1.jpg
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
Anti-VWF antibody, ASA-B1983--2.jpg
All lanes: Anti-VWF(ASA-B1983) at 0.5ug/ml
WB: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 309KD
Observed bind size: 309KD
All lanes: Anti-VWF(ASA-B1983) at 0.5ug/ml
WB: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 309KD
Observed bind size: 309KD
