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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| vinculin | Polyclonal | IgG | Rabbit | Mouse | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Vinculin(173-188aa KMIDERQQELTHQEHR), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | VCL |
Protein | Vinculin |
Uniprot ID | P18206 |
Function | Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. |
Tissue Specificity | Metavinculin is muscle-specific. |
Sub-cellular localization | Cytoplasm, cytoskeleton. |
Sequence Similarities | Belongs to the vinculin/alpha-catenin family. |
Aliases | CMD1W antibody|CMH15 antibody|Metavinculin antibody|MVCL antibody|OTTHUMP00000019861 antibody|OTTHUMP00000019862 antibody|VCL antibody|VINC antibody|VINC_HUMAN antibody|Vinculin antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Vinculin antibody, ASA-B1977, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Liver Tissue Lysate
Lane 4: U87 Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 6: HEPA Cell Lysate
Lane 7: HELA Cell Lysate
Lane 8: HT1080 Cell Lysate
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Liver Tissue Lysate
Lane 4: U87 Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 6: HEPA Cell Lysate
Lane 7: HELA Cell Lysate
Lane 8: HT1080 Cell Lysate
Anti-Vinculin antibody, ASA-B1977, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
Anti-Vinculin antibody, ASA-B1977, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue