More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| vimentin | Monoclonal | IgG | Mouse | Human, Pig, Rabbit, Rat | IHC-P, WB | Ascites |
Immunogen |
| Pig eye lens vimentin. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | VIM |
Protein | Vimentin |
Uniprot ID | Human(P08670), Mouse(P20152), Rat(P31000) |
Function | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. |
Tissue Specificity | Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the intermediate filament family. |
Aliases | |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.5-1μg/ml | Human, rabbit, pig, rat. | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 1.5-2.5μg/ml | Human, rabbit, pig, rat. | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Vimentin antibody (monoclonal), ASA-B1974, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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