More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| versican | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Versican (65-92aa KWSKIEVDKNGKDLKETTVLVAQNGNIK), different from the related mouse and rat sequences by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | VCAN |
Protein | Versican core protein |
Uniprot ID | P13611 |
Function | May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid. |
Tissue Specificity | Cerebral white matter and plasma. Isoform V0 and isoform V1 are expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2 is restricted to normal brain and gliomas. Isoform V3 is found in all these tissues except medulloblastomas. |
Sub-cellular localization | Secreted, extracellular space, extracellular matrix. |
Sequence Similarities | Belongs to the aggrecan/versican proteoglycan family. |
Aliases | Chondroitin sulfate proteoglycan 2 antibody|Chondroitin sulfate proteoglycan core protein 2 antibody|CSPG2 antibody|CSPG2_HUMAN antibody|ERVR antibody| GHAP antibody|Glial hyaluronate binding protein antibody|Glial hyaluronate-binding protein antibody|Large fibroblast proteoglycan antibody|PG-M antibody| PGM antibody|VCAN antibody|Versican core protein antibody|Versican proteoglycan antibody|WGN 1 antibody|WGN antibody|WGN1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Versican antibody, ASA-B1971, Western blotting
All lanes: Anti Versican (ASA-B1971) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 373KD
Observed bind size: 373KD
All lanes: Anti Versican (ASA-B1971) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 373KD
Observed bind size: 373KD
Anti- Versican antibody, ASA-B1971, IHC(P)
IHC(P): Human Glioma Tissue
IHC(P): Human Glioma Tissue
