VEGF Receptor 2 Antibody View larger

VEGF Receptor 2 Antibody

ASA-B1967

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

kinase insert domain receptor(a type III receptor tyrosine kinase) Polyclonal IgG Rabbit Human IHC-P, IHC-F, ICC, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human VEGF Receptor 2(454-469aa HHIHWYWQLEEECANE).

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

KDR

Protein

Vascular endothelial growth factor receptor 2

Uniprot ID

P35968

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.

Tissue Specificity

Detected in cornea (at protein level). Widely expressed.

Sub-cellular localization

Cell junction . Endoplasmic reticulum.

Sequence Similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

Aliases

CD309 antibody|CD309 antigen antibody|Fetal liver kinase 1 antibody|FLK-1 antibody|FLK1 antibody|Kdr antibody|Kinase insert domain receptor(a type III receptor tyrosine kinase) antibody|Kinase insert domain receptor antibody|KRD1 antibody|Ly73 antibody|Protein tyrosine kinase receptor FLK1 antibody|Protein-tyrosine kinase receptor flk-1 antibody|Tyrosine kinase growth factor receptor antibody|Vascular endothelial growth factor receptor 2 antibody|VEGFR 2 antibody|VEGFR antibody|VEGFR-2 antibody|VEGFR2 antibody|VGFR2_HUMAN antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml HumanAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section) 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit
Immunocytochemistry 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-VEGF Receptor 2 antibody, ASA-B1967, Western blotting
WB: SMMC Cell Lysate

Anti-VEGF Receptor 2 antibody, ASA-B1967, IHC(P)
IHC(P): Human Lung Cancer Tissue
Anti-VEGF Receptor 2 antibody, ASA-B1967, ICC
ICC: HELA Cell

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