More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| vitamin D (1,25- dihydroxyvitamin D3) receptor | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human VDR (377-402aa HLLYAKMIQKLADLRSLNEEHSKQYR), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | VDR |
Protein | Vitamin D3 receptor |
Uniprot ID | P11473 |
Function | Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Recruited to promoters via its interaction with BAZ1B/WSTF which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the nuclear hormone receptor family. NR1 subfamily. |
Aliases | 1 25 dihydroxyvitamin D3 receptor antibody|1 antibody|1,25-@dihydroxyvitamin D3 receptor antibody|125 dihydroxyvitamin D3 receptor antibody|25-dihydroxyvitamin D3 receptor antibody|Member 1 antibody|NR1I1 antibody|Nuclear receptor subfamily 1 group I member 1 antibody|VDR antibody|VDR_HUMAN antibody| Vitamin D (1,25- dihydroxyvitamin D3) receptor antibody|Vitamin D hormone receptor antibody|Vitamin D nuclear receptor variant 1 antibody| Vitamin D receptor antibody|Vitamin D3 receptor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-VDR antibody, ASA-B1960, Western blotting
All lanes: Anti VDR (ASA-B1960) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Rat Pancreas Tissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Predicted bind size: 48KD
Observed bind size: 48KD
All lanes: Anti VDR (ASA-B1960) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Rat Pancreas Tissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Predicted bind size: 48KD
Observed bind size: 48KD
Anti-VDR antibody, ASA-B1960, IHC(P)
IHC(P): Mouse Intestine Tissue
IHC(P): Mouse Intestine Tissue
Anti-VDR antibody, ASA-B1960, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue