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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| voltage-dependent anion channel 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human VDAC(163-178aa RVTQSNFAVGYKTDEF), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | VDAC1 |
Protein | Voltage-dependent anion-selective channel protein 1(VDAC-1/hVDAC1) |
Uniprot ID | P21796 |
Function | Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis. |
Tissue Specificity | Heart, liver and skeletal muscle. |
Sub-cellular localization | Mitochondrion outer membrane. |
Sequence Similarities | Belongs to the eukaryotic mitochondrial porin family. |
Aliases | N2441 antibody|OMP2 antibody|POR1 antibody|hVDAC1 antibody|MGC111064 antibody|Mitochondrial Porin antibody|Outer mitochondrial membrane protein porin 1 antibody|Plasmalemmal porin antibody|Porin 31 HL antibody|Porin 31HL antibody|Porin 31HM antibody|VDAC 1 antibody|VDAC antibody|VDAC-1 antibody|VDAC1 antibody|VDAC1_HUMAN antibody|Voltage dependent anion channel 1 antibody|Voltage dependent anion selective channel protein 1 antibody|Voltage-dependent anion-selective channel protein 1 antibody|YNL055C antibody|YNL2441C antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human, Mouse Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-VDAC/Porin antibody, ASA-B1958, Western blotting
All lanes: Anti VDAC/Porin (ASA-B1958) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Rat Heart Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: A431 Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: SMMC Whole Cell Lysate at 40ug
Lane 8: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 31KD
All lanes: Anti VDAC/Porin (ASA-B1958) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Rat Heart Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: A431 Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: SMMC Whole Cell Lysate at 40ug
Lane 8: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 31KD
Anti-VDAC/Porin antibody, ASA-B1958, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
IHC(P): Rat Cardiac Muscle Tissue
Anti-VDAC/Porin antibody, ASA-B1958, IHC(P)
IHC(P): Rat Skeletal Muscle Tissue
IHC(P): Rat Skeletal Muscle Tissue