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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| unc-51 like kinase 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at C-terminus of human ULK3 (427-454aa HTEVQNLMARAEYLKEQVKMRESRWEAD), different from the related mouse and rat sequences by four amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ULK3 |
Protein | Serine/threonine-protein kinase ULK3 |
Uniprot ID | Q6PHR2 |
Function | Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy. |
Tissue Specificity | Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optic nerve. In the central nervous system, lowest levels in the spinal cord. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. |
Aliases | DKFZP434C131 antibody|FLJ90566 antibody|Serine/threonine-protein kinase ULK3 antibody|Ulk3 antibody|ULK3_HUMAN antibody|unc 51 like kinase 3 (C. elegans) antibody|Unc-51-like kinase 3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- ULK3 antibody, ASA-B1951, Western blotting
All lanes: Anti ULK3 (ASA-B1951) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Testis Tissue Lysate at 50ug
Lane 3: Mouse Brain Tissue Lysate at 50ug
Lane 4: Human Placenta Tissue Lysate at 50ug
Lane 5: 22RV1 Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 53KD
Observed bind size: 53KD
All lanes: Anti ULK3 (ASA-B1951) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Testis Tissue Lysate at 50ug
Lane 3: Mouse Brain Tissue Lysate at 50ug
Lane 4: Human Placenta Tissue Lysate at 50ug
Lane 5: 22RV1 Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 53KD
Observed bind size: 53KD
Anti- ULK3 antibody, ASA-B1951, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
Anti- ULK3 antibody, ASA-B1951, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue