More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| thioredoxin reductase 2 | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human TXNRD2(418-434aa RHGQEHVEVYHAHYKPL), different from the related rat sequence by three amino acids, and from the related mouse sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TXNRD2 |
Protein | Thioredoxin reductase 2, mitochondrial |
Uniprot ID | Q9NNW7 |
Function | Maintains thioredoxin in a reduced state. Implicated in the defenses against oxidative stress. May play a role in redox- regulated cell signaling. |
Tissue Specificity | Highly expressed in the prostate, ovary, liver, testis, uterus, colon and small intestine. Intermediate levels in brain, skeletal muscle, heart and spleen. Low levels in placenta, pancreas, thymus and peripheral blood leukocytes. According to PubMed:10608886, high levels in kidney, whereas according to PubMed:9923614, levels are low. |
Sub-cellular localization | Mitochondrion. |
Sequence Similarities | Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. |
Aliases | mitochondrial antibody|selenoprotein Z antibody|SELZ antibody|Thioredoxin reductase 2 antibody|Thioredoxin reductase 2 mitochondrial antibody|thioredoxin reductase 3 antibody|thioredoxin reductase beta antibody|Thioredoxin reductase TR3 antibody|TR 3 antibody|TR antibody|TR beta antibody|TR-beta antibody|TR3 antibody|TRXR 2 antibody|TRXR2 antibody|TRXR2_HUMAN antibody|TXNRD 2 antibody|Txnrd2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TXNRD2 antibody, ASA-B1944, Western blotting
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Ovary Tissue Lysate
Lane 3: Rat Liver Tissue Lysate
Lane 4: SMMC Cell Lysate
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Ovary Tissue Lysate
Lane 3: Rat Liver Tissue Lysate
Lane 4: SMMC Cell Lysate
Anti-TXNRD2 antibody, ASA-B1944, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
