More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| tuberous sclerosis 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Tuberin recombinant protein (Position: H1611-V1807). Human Tuberin shares 94% and 90% amino acid (aa) sequences identity with mouse and rat Tuberin, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TSC2 |
Protein | Tuberin |
Uniprot ID | P49815 |
Function | In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase- activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors. |
Tissue Specificity | Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta. |
Sub-cellular localization | Cytoplasm. Membrane; Peripheral membrane protein. Note: At steady state found in association with membranes. |
Sequence Similarities | Contains 1 Rap-GAP domain. |
Aliases | FLJ43106 antibody|LAM antibody|OTTHUMP00000158940 antibody|OTTHUMP00000198394 antibody|OTTHUMP00000198395 antibody|TSC2 antibody|TSC2_HUMAN antibody|TSC4 antibody|TSC4 gene, formerly antibody|TSC4, formerly antibody|Tuberin antibody|Tuberous sclerosis 2 antibody|Tuberous sclerosis 2 protein antibody|Tuberous sclerosis 2 protein homolog antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Tuberin antibody, ASA-B1943--1.jpg
All lanes: Anti Tuberin (ASA-B1943) at 0.5ug/ml
WB: Recombinant Human Tuberin Protein 0.5ng
Predicted bind size: 41KD
Observed bind size: 41KD
All lanes: Anti Tuberin (ASA-B1943) at 0.5ug/ml
WB: Recombinant Human Tuberin Protein 0.5ng
Predicted bind size: 41KD
Observed bind size: 41KD
Anti-Tuberin antibody, ASA-B1943--2.jpg
All lanes: Anti Tuberin (ASA-B1943) at 0.5ug/ml
Lane 1: U20S Whole Cell Lysate at 40ug
Lane 2: PANC Whole Cell Lysate at 40ug
Lane 3: HEPG2 Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 201KD
Observed bind size: 201KD
All lanes: Anti Tuberin (ASA-B1943) at 0.5ug/ml
Lane 1: U20S Whole Cell Lysate at 40ug
Lane 2: PANC Whole Cell Lysate at 40ug
Lane 3: HEPG2 Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 201KD
Observed bind size: 201KD
Anti-Tuberin antibody, ASA-B1943--3.jpg
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue