More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| transient receptor potential cation channel, subfamily M, member 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human TRPM2(91-106aa CGYTHEQHLEEATKPH), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TRPM2 |
Protein | Transient receptor potential cation channel subfamily M member 2 |
Uniprot ID | O94759 |
Function | Nonselective, voltage-independent cation channel mediating sodium and calcium ion influx in response to oxidative stress. Extracellular calcium passes through the channel and acts from the intracellular side as a positive regulator in channel activation. Activated by ADP-ribose, nicotinamide adenine dinucleotide (NAD(+)), reactive nitrogen species and arachidonic acid. Inactivated by intracellular ATP. Confers susceptibility to cell death following oxidative stress. Isoform 2 does not seem to be regulated by ADPR. Has ADP-ribose pyrophosphatase activity. |
Tissue Specificity | Highly expressed in brain and peripheral blood cells, such as neutrophils. Also detected in bone marrow, spleen, heart, liver and lung. Isoform 2 is found in neutrophil granulocytes. |
Sub-cellular localization | Membrane. |
Sequence Similarities | Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM2 sub-subfamily. |
Aliases | EREG 1 antibody|EREG1 antibody|Estrogen responsive element associated gene 1 antibody|Estrogen responsive element associated gene 1 protein antibody|Estrogen-responsive element-associated gene 1 protein antibody|KNP 3 antibody|KNP3 antibody|Long transient receptor potential channel 2 antibody|LTRPC 2 antibody|LTrpC-2 antibody|LTRPC2 antibody|MGC133383 antibody|NUDT9H antibody|NUDT9L1 antibody|putative Ca2+ channel protein antibody|transient receptor potential cation channel subfamily M member 2 antibody|Transient receptor potential channel 7 antibody|TRPC 7 antibody|TRPC7 antibody|TRPM 2 antibody|Trpm2 antibody|TRPM2_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TRPM2 antibody, ASA-B1934, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
Lane 3: Mouse Liver Tissue Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: HELA Cell Lysate
Lane 6: A549 Cell Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
Lane 3: Mouse Liver Tissue Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: HELA Cell Lysate
Lane 6: A549 Cell Lysate
Anti-TRPM2 antibody, ASA-B1934, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
Anti-TRPM2 antibody, ASA-B1934, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue