More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| transient receptor potential cation channel, subfamily C, member 6 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human TRPC6(249-265aa HDYFCKCNDCNQKQKHD), different from the related rat and mouse sequences by three amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TRPC6 |
Protein | Short transient receptor potential channel 6(TrpC6) |
Uniprot ID | Q9Y210 |
Function | Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion. |
Tissue Specificity | Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm. |
Sub-cellular localization | Membrane ; Multi-pass membrane protein . |
Sequence Similarities | Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC6 sub-subfamily. |
Aliases | bZ1P14.9 antibody|FLJ11098 antibody|FLJ14863 antibody|FSGS2 antibody|MTRP6 antibody|Short transient receptor potential channel 6 antibody|si:rp71-1p14.9 antibody|Transient Receptor Potential Cation Channel Subfamily C Member 6 antibody|Transient receptor protein 6 antibody|TRP 6 antibody|TRP-6 antibody|TRP6 antibody|TRPC 6 antibody|TrpC6 antibody|Trpc6 antibody|TRPC6_HUMAN antibody|TRRP6 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human, Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TRPC6 antibody, ASA-B1933, Western blotting
Lane 1: Rat Lung Tissue Lysate
Lane 2: 293T Cell Lysate
Lane 3: 293T Cell Lysate
Lane 1: Rat Lung Tissue Lysate
Lane 2: 293T Cell Lysate
Lane 3: 293T Cell Lysate
Anti-TRPC6 antibody, ASA-B1933, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
Anti-TRPC6 antibody, ASA-B1933, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue