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TRIF Antibody

ASA-B1916

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
toll-like receptor adaptor molecule 1 Polyclonal IgG Rabbit Human IHC-P, IHC-F, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human TRIF(692-712aa NNHMWNQRGSQAPEDKTQEAE).

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 TICAM1

Protein

 TIR domain-containing adapter molecule 1

Uniprot ID

 Q8IUC6

Function

 Involved in innate immunity against invading pathogens. Adapter used by TLR3 and TLR4 (through TICAM2) to mediate NF- kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein- interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively.

Tissue Specificity

Ubiquitously expressed but with higher levels in liver.

Sub-cellular localization

 Cytoplasmic vesicle, autophagosome.

Sequence Similarities

 Contains 1 TIR domain.

Aliases

 HSD34 antibody|MGC35334 antibody|Proline rich vinculin and TIR domain containing protein B antibody|Proline-rich antibody|PRVTIRB antibody|Putative NF kappa B activating protein 502H antibody|Putative NF-kappa-B-activating protein 502H antibody|Putative NFkB activating protein antibody|Ring finger protein 36 antibody|RNF36 antibody|TCAM1_HUMAN antibody|TICAM 1 antibody|TICAM-1 antibody|TICAM1 antibody|TIR domain containing adapter molecule 1 antibody|TIR domain containing adapter protein inducing IFN beta antibody|TIR domain containing adaptor inducing interferon beta antibody|TIR domain-containing adapter molecule 1 antibody|TIR domain-containing adapter protein inducing IFN-beta antibody|Toll interleukin 1 receptor domain containing adapter protein inducing interferon beta antibody|Toll like receptor adaptor molecule 1 antibody|Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta antibody|TRIF antibody|TRIF protein antibody|TRIM 69 antibody|TRIM69 antibody|Trimless antibody|Tripartite motif containing 69 antibody|vinculin and TIR domain-containing protein B antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml HumanAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section) 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TRIF antibody, ASA-B1916, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human TICAM1, 44.7KD(162aa tag+ Q468-E712)
Lane 1: Recombinant Human TICAM1 Protein 10ng
Lane 2: Recombinant Human TICAM1 Protein 5ng
Lane 3: Recombinant Human TICAM1 Protein 2.5ng
Lane 4: Recombinant Human TICAM1 Protein 1.25ng
Anti-TRIF antibody, ASA-B1916, Western blotting
Lane 1: JURKAT Cell Lysate
Lane 2: HL-60 Cell Lysate
Anti-TRIF antibody, ASA-B1916, IHC(P)
IHC(P): Human Mammary Cancer Tissue

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