More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| translocation associated membrane protein 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human TRAM1(314-331aa KFINFQLRRWREHSAFQA), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TRAM1 |
Protein | Translocating chain-associated membrane protein 1 |
Uniprot ID | Q15629 |
Function | Stimulatory or required for the translocation of secretory proteins across the ER membrane. |
Tissue Specificity | |
Sub-cellular localization | Endoplasmic reticulum membrane; Multi-pass membrane protein. |
Sequence Similarities | Belongs to the TRAM family. |
Aliases | PNAS 8 antibody|PNAS8 antibody|PRO1292 antibody|TRAM 1 antibody|TRAM antibody|TRAMP antibody|Translocating chain associated membrane protein 1 antibody|Translocating chain associating membrane protein antibody|Translocation associated membrane protein 1 antibody|Translocation associating membrane protein 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F)and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TRAM1 antibody, ASA-B1910, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: 293T Cell Lysate
Lane 4: RAJI Cell Lysate
Lane 5: JURKAT Cell Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: 293T Cell Lysate
Lane 4: RAJI Cell Lysate
Lane 5: JURKAT Cell Lysate
Anti-TRAM1 antibody, ASA-B1910, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
Anti-TRAM1 antibody, ASA-B1910, IHC(P)
IHC(P): Rat Kidney Tissue
IHC(P): Rat Kidney Tissue