More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| tumor necrosis factor(ligand) superfamily, member 10 | Polyclonal | IgG | Rabbit | Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse TRAIL(272-291aa NEHLMDLDQEASFFGAFLIN), different from the related rat sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TNFSF10 |
Protein | Tumor necrosis factor ligand superfamily member 10 |
Uniprot ID | P50592 |
Function | Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. |
Tissue Specificity | Widespread. |
Sub-cellular localization | Membrane ; Single-pass type II membrane protein . |
Sequence Similarities | |
Aliases | Apo 2 ligand antibody|Apo 2L antibody|Apo-2 ligand antibody|Apo-2L antibody|APO2L antibody|CD 253 antibody|CD253 antibody|CD253 antigen antibody|Chemokine tumor necrosis factor ligand superfamily member 10 antibody|Protein TRAIL antibody|TL 2 antibody|TL2 antibody|TNF Related Apoptosis Inducing Ligand antibody|TNF related apoptosis inducing ligand TRAIL antibody|TNF-related apoptosis-inducing ligand antibody|TNF10_HUMAN antibody|TNFSF 10 antibody|TNFSF10 antibody|TNFSF10 protein antibody|TRAIL antibody|TRAIL PEN antibody|Tumor necrosis factor(ligand) family member 10 antibody|Tumor Necrosis Factor(ligand) Superfamily Member 10 antibody|Tumor necrosis factor apoptosis inducing ligand splice variant delta antibody|Tumor necrosis factor ligand superfamily member 10 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Mouse, Rat Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TRAIL antibody, ASA-B1908, Western blotting
Lane 1: Recombinant Mouse Trail Protein 10ng
Lane 2: Recombinant Mouse Trail Protein 5ng
Lane 3: Recombinant Mouse Trail Protein 2ng
Lane 1: Recombinant Mouse Trail Protein 10ng
Lane 2: Recombinant Mouse Trail Protein 5ng
Lane 3: Recombinant Mouse Trail Protein 2ng
