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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| TNF receptor-associated factor 4 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human TRAF4(437-454aa PKFISHQDIRKRNYVRDD), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TRAF4 |
Protein | TNF receptor-associated factor 4 |
Uniprot ID | Q9BUZ4 |
Function | Adapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract (By similarity). Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions. |
Tissue Specificity | Expressed in epithelial cells of thymus, dendritic cells of lymph node, and in the basal cell layer of epithelia such as epidermis, nasopharynx, respiratory tract, salivary gland, and esophagus. |
Sub-cellular localization | Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Cell junction, tight junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton . |
Sequence Similarities | Belongs to the TNF receptor-associated factor family. B subfamily. |
Aliases | CART 1 antibody|CART1 antibody|cysteine rich domain associated with ring and TRAF domain antibody|Cysteine rich domain associated with RING and Traf domains protein 1 antibody|Cysteine-rich domain associated with RING and Traf domains protein 1 antibody|Malignant 62 antibody|metastatic lymph node gene 62 protein antibody|MLN 62 antibody|MLN62 antibody|RING finger protein 83 antibody|RNF 83 antibody|RNF83 antibody|TNF receptor associated factor 4 antibody|TNF receptor-associated factor 4 antibody|TRAF 4 antibody|Traf4 antibody|TRAF4_HUMAN antibody|tumor necrosis receptor-associated factor 4A antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TRAF4 antibody, ASA-B1904, Western blotting
Lane 1: Rat Thymus Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: JURKAT Cell Lysate
Lane 4: HEPA Cell Lysate
Lane 1: Rat Thymus Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: JURKAT Cell Lysate
Lane 4: HEPA Cell Lysate
Anti-TRAF4 antibody, ASA-B1904, IHC(P)
IHC(P): Rat Lymphonodus Tissue
IHC(P): Rat Lymphonodus Tissue
Anti-TRAF4 antibody, ASA-B1904, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue