More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| toll-like receptor 5 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human TLR5 (801-828aa MKHQSIRGFVQKQQYLRWPEDFQDVGWF), different from the related mouse sequence by five amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TLR5 |
Protein | Toll-like receptor 5 |
Uniprot ID | O60602 |
Function | Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. |
Tissue Specificity | Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis. |
Sub-cellular localization | Membrane ; Single-pass type I membrane protein . |
Sequence Similarities | Belongs to the Toll-like receptor family. |
Aliases | FLJ10052 antibody|MGC126430 antibody|MGC126431 antibody|SLEB1 antibody| TIL 3 antibody|TIL3 antibody|TLR 5 antibody|Tlr5 antibody|TLR5_HUMAN antibody| Toll like receptor 5 antibody| Toll like receptor 5 precursor antibody|Toll-like receptor 5 antibody|Toll/interleukin 1 receptor like protein 3 antibody| Toll/ interleukin-1 receptor-like protein 3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- TLR5 antibody, ASA-B1875, Western blotting
All lanes: Anti TLR5 (ASA-B1875) at 0.5ug/ml
WB: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 98KD
Observed bind size: 98KD
All lanes: Anti TLR5 (ASA-B1875) at 0.5ug/ml
WB: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 98KD
Observed bind size: 98KD
Anti- TLR5 antibody, ASA-B1875, IHC(P)
IHC(P): Human Appendicitis Tissue
IHC(P): Human Appendicitis Tissue
