More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| tight junction protein 2 | Polyclonal | IgG | Rabbit | Human | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human TJP2(1171-1190aa SEHSKRGYYGQSARYRDTEL). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TJP2 |
Protein | Tight junction protein ZO-2 |
Uniprot ID | Q9UDY2 |
Function | Plays a role in tight junctions and adherens junctions. |
Tissue Specificity | This protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. Not detected in brain and skeletal muscle. Found in normal as well as in most neoplastic tissues. |
Sub-cellular localization | Cell junction, adherens junction. Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, tight junction . Nucleus . Note: Also nuclear under environmental stress conditions and in migratory endothelial cells and subconfluent epithelial cell cultures. |
Sequence Similarities | Belongs to the MAGUK family. |
Aliases | Friedreich ataxia region gene X104(tight junction protein ZO-2) antibody|MGC26306 antibody|Tight junction protein 2 antibody|Tight junction protein ZO 2 antibody|Tight junction protein ZO-2 antibody|TJP2 antibody|X104 antibody|ZO 2 antibody|ZO-2 antibody|ZO2 antibody|ZO2_HUMAN antibody|Zona occludens protein 2 antibody|Zonula occludens protein 2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TJP2 antibody, ASA-B1868, Western blotting
Lane 1: 293T Cell Lysate
Lane 2: MCF-7 Cell Lysate
Lane 1: 293T Cell Lysate
Lane 2: MCF-7 Cell Lysate
Anti-TJP2 antibody, ASA-B1868, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
