More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| tight junction protein 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human TJP1 recombinant protein (Position: H1178-F1527). Human TJP1 shares 82% amino acid (aa) sequence identity with mouse TJP1. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TJP1 |
Protein | Tight junction protein ZO-1 |
Uniprot ID | Q07157 |
Function | The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. |
Tissue Specificity | The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. |
Sub-cellular localization | Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, tight junction. Cell junction. Cell junction, gap junction. Note: Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact. Detected at the leading edge of migrating and wounded cells. |
Sequence Similarities | Belongs to the MAGUK family. |
Aliases | Tight junction protein 1 antibody|Tight junction protein ZO-1 antibody|Tight junction protein ZO1 antibody|Tjp1 antibody|zo-1 antibody|Zo1 antibody|ZO1_HUMAN antibody|Zona occludens 1 antibody|Zona occludens 1 protein antibody|Zona occludens protein 1 antibody|Zonula occludens 1 protein antibody|Zonula occludens protein 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- TJP1 antibody, ASA-B1867, Western blotting
All lanes: Anti TJP1 (ASA-B1867) at 0.5ug/ml
WB: Recombinant Human TJP1 Protein 0.5ng
Predicted bind size: 48KD
Observed bind size: 48KD
All lanes: Anti TJP1 (ASA-B1867) at 0.5ug/ml
WB: Recombinant Human TJP1 Protein 0.5ng
Predicted bind size: 48KD
Observed bind size: 48KD
Anti- TJP1 antibody, ASA-B1867, Western blotting
All lanes: Anti TJP1 (ASA-B1867) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Lane 3: NRK Whole Cell Lysate at 40ug
Lane 4: PC-12 Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Lane 7: HEPA Whole Cell Lysate at 40ug
Lane 8: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 195KD
Observed bind siz
All lanes: Anti TJP1 (ASA-B1867) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Lane 3: NRK Whole Cell Lysate at 40ug
Lane 4: PC-12 Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Lane 7: HEPA Whole Cell Lysate at 40ug
Lane 8: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 195KD
Observed bind siz
Anti- TJP1 antibody, ASA-B1867, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue