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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| TIMP metallopeptidase inhibitor 4 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human TIMP4(208-224aa YRGHLPLRKEFVDIVQP), different from the related rat sequence by two amino acids, and from the related mouse sequence by three amino acids. | ||||||
Properties
Form | Liquid |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 50% glycerol, 0.9 mg NaCl, 0.2 mg Na2HPO4. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied, at 2-8 °C for one month. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TIMP4 |
Protein | Metalloproteinase inhibitor 4 |
Uniprot ID | Q99727 |
Function | Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP- 9. |
Tissue Specificity | Abundant in heart and present at low levels in many other tissues. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the protease inhibitor I35 (TIMP) family. |
Aliases | Metalloproteinase inhibitor 4 antibody|TIMP 4 antibody|TIMP metallopeptidase inhibitor 4 antibody|TIMP-4 antibody|Timp4 antibody|TIMP4_HUMAN antibody|Tissue inhibitor of metalloproteinase 4 antibody|Tissue inhibitor of metalloproteinases 4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TIMP4 antibody, ASA-B1866, Western blotting
All lanes: Anti TIMP4 (ASA-B1866) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 25KD
Observed bind size: 25KD
All lanes: Anti TIMP4 (ASA-B1866) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 25KD
Observed bind size: 25KD
Anti-TIMP4 antibody, ASA-B1866, IHC(P)
IHC(P): Rat Kidney Tissue
IHC(P): Rat Kidney Tissue
Anti-TIMP4 antibody, ASA-B1866, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue