More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| thrombospondin 2 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Thrombospondin 2(637-655aa EAAKTEKQVCEPENPCKDK), different from the mouse sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | THBS2 |
Protein | Thrombospondin-2 |
Uniprot ID | P35442 |
Function | Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties. |
Tissue Specificity | High expression in invertebral disk tissue. |
Sub-cellular localization | |
Sequence Similarities | Belongs to the thrombospondin family. |
Aliases | THBS2 antibody|Thrombospondin-2 antibody|TSP2 antibody|TSP2_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Thrombospondin 2 antibody, ASA-B1857, IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue
Anti-Thrombospondin 2 antibody, ASA-B1857, Western blotting
All lanes: Anti THBS2 (ASA-B1857) at 0.5ug/ml
Lane 1: SMMC Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: HT1080 Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Lane 5: NIH 3T3 Whole Cell Lysate at 40ug
Predicted bind size: 130KD
Observed bind size: 130KD
All lanes: Anti THBS2 (ASA-B1857) at 0.5ug/ml
Lane 1: SMMC Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: HT1080 Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Lane 5: NIH 3T3 Whole Cell Lysate at 40ug
Predicted bind size: 130KD
Observed bind size: 130KD
