More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| transforming growth factor, beta receptor 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human TGF beta Receptor I(281-295aa DYHEHGSLFDYLNRY), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TGFBR1 |
Protein | TGF-beta receptor type-1(TGFR-1) |
Uniprot ID | P36897 |
Function | Transmembrane serine/threonine kinase forming with the TGF-beta type II serine/threonine kinase receptor, TGFBR2, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non- canonical, SMAD-independent TGF-beta signaling pathways. For instance, TGFBR1 induces TRAF6 autoubiquitination which in turn results in MAP3K7 ubiquitination and activation to trigger apoptosis. Also regulates epithelial to mesenchymal transition through a SMAD-independent signaling pathway through PARD6A phosphorylation and activation. |
Tissue Specificity | Found in all tissues examined, most abundant in placenta and least abundant in brain and heart. |
Sub-cellular localization | Cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. |
Sequence Similarities | Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily. |
Aliases | AAT 5 antibody|AAT5 antibody|Activin A receptor type II like kinase 53kDa antibody|Activin A receptor type II like kinase, 53kD antibody|Activin A receptor type II like protein kinase of 53kD antibody|activin A receptor type II-like kinase, 53kDa antibody|activin A receptor type II-like protein kinase of 53kD antibody|Activin receptor like kinase 5 antibody|Activin receptor-like kinase 5 antibody|ACVRLK 4 antibody|ACVRLK4 antibody|ALK 5 antibody|ALK-5 antibody|ALK5 antibody|LDS1A antibody|LDS2A antibody|MSSE antibody|Serine/threonine protein kinase receptor R4 antibody|Serine/threonine-protein kinase receptor R4 antibody|SKR 4 antibody|SKR4 antibody|TbetaR I antibody|TbetaR-I antibody|TGF beta receptor type 1 antibody|TGF beta receptor type I antibody|TGF beta type I receptor antibody|TGF-beta receptor type I antibody|TGF-beta receptor type-1 antibody|TGF-beta type I receptor antibody|TGFBR 1 antibody|TGFBR1 antibody|TGFBR1 protein antibody|TGFR 1 antibody|TGFR-1 antibody|TGFR1 antibody|TGFR1_HUMAN antibody|Transforming growth factor beta receptor 1 antibody|Transforming growth factor beta receptor I(activin A receptor type II like kinase, 53kD) antibody|Transforming growth factor beta receptor I antibody|transforming growth factor, beta receptor 1 antibody|transforming growth factor, beta receptor I(activin A receptor type II-like kinase, 53kD) antibody|Transforming growth factor-beta receptor type I antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TGF beta Receptor I antibody, ASA-B1851, Western blotting
All lanes: Anti TGF beta Receptor I (ASA-B1851) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 56KD
Observed bind size: 56KD
All lanes: Anti TGF beta Receptor I (ASA-B1851) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 56KD
Observed bind size: 56KD
Anti-TGF beta Receptor I antibody, ASA-B1851, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
